Animals models for studying diabetes mellitus etuk, e. Evaluation of antidiabetic activity of ethanolic extract. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland. The epidemic of type 2 diabetes mellitus t2dm is fueled by added fructose consumption. Diabetes was induced by intravenously injecting streptozotocin 55mgkg in 0. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Fvbnj 001800, balbcj 000651, and aj 000646 males are resistant. Increased plasma adiponectin levels have been reported in type 1 diabetic patients, however studies. Original article effects of panax quinquefolium on. The males of various strains are differentially susceptible to developing diabetes.
Impact of streptozotocininduced diabetes and insulin. The booklet describes gestational diabetes, its causes, and its features and. Pdf induction of diabetes by streptozotocin in rats. Mccabe abstract as many as 50% of adults with type i t1 diabetes exhibit bone loss and are at increased risk for fractures. The streptozotocindiabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. Ebscohost serves thousands of libraries with premium essays, articles and other content including induction of diabetes mellitus in rats using intraperitoneal streptozotocin. Sep 01, 2015 streptozotocin stz is an antibiotic that produces pancreatic islet. In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Some reports suggest cellular toxicity outside of the pancreas. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural.
The mechanism of alloxan and streptozotocin action in b cells. Unlike type 1 diabetes, type 2 diabetes t2d is associated with resistance to insulin action. Hyperglycemia enhances the production of reactive oxygen species. As a general rule, there is no need to avoid alcohol because you have diabetes. Stz is toxic to the insulinproducing beta cells of the pancreas and used to induce a diabetes similar to a type i diabetic reference. Streptozotocin is a glucosaminenitrosourea compound. U department of pharmacology, college of health sciences, usmanu danfodiyo university, sokoto, nigera phone. Evaluation of antidiabetic activity of ethanolic extract of. Diabetes was induced in rats by single intraperitoneal injection of streptozotocin 45mgkg body weight. Stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. The mechanisms of alloxan and streptozotocininduced diabetes.
Induction of diabetes mellitus in rats using intraperitoneal. Impact of streptozotocin induced diabetes and insulin therapy on plasma adiponectin levels adiponectin also known as acr adiponectin also known as acrp 30, adipoq, and gbp28 is an adipokine implicated in the regulation of insulin sensitivity. Changes in oxidative stress and antioxidant enzyme activities. It means your blood glucose levels are higher than normal but arent high enough to be called diabetes. The mechanisms of alloxan and streptozotocininduced. Therapeutic development to prevent bone loss andor restore lost bone in t1 diabetic patients requires. Multiple low dose streptozotocininduced diabetes in the. The ethanolic extract of alternanthera sessilis linn. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by the glucose tolerance test.
Cdcs division of diabetes translation offers many fact sheets about diabetes. Impact of streptozotocininduced diabetes and insulin therapy on plasma adiponectin levels adiponectin also known as acr adiponectin also known as acrp 30, adipoq, and gbp28 is an adipokine implicated in the regulation of insulin sensitivity. Full text streptozotocininduced type 1 diabetes in rodents. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and house in a 12h light, 12h dark cycle environment, temperature 25c, with standard rat diet and water in metabolic cages for one week prior to. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Protective role of opn in the islets osteopontin opn is a secrete osteopontin opn is a secreted acidic phosphoprotein that binds to a cell surface integrinbinding motif and is involved in many inflammatory and immunemodulating disorders. Diabetes handouts for providers and educators scripps health. As many as 50% of adults with type i t1 diabetes exhibit bone loss and are at increased risk for fractures. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. The responses are accompanied by corresponding inverse changes in.
Furthermore explore the role of age in cpeptide and nitric oxide no and their relation in stz induced diabetic rats. The streptozotocin induced diabetic rats showed significant reductions in biochemical parameter after treatment with the extract and glibenclamide used as standard as compared to the diabetic controls. A comparison between 2 strains of rats article pdf available in european journal of scientific research 323. Evaluation of antidiabetic activity of ethanolic extract of alternanthera sessilis linn. Streptozotocininduced diabetic models in mice and rats. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. Multiple low dose streptozotocininduced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Streptozotocin stz is an antibiotic that produces pancreatic islet. In streptozotocin and highfat diet induced diabetes model of mice, it is shown that ursolic acid protected pancreatic. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. We evaluated nude male mice from various sources for their sensitivity to a single high dose 160 to 240 mgkg of stz.
Three days after degeneration of beta cells, diabetes was induced in all animals. Key words streptozotocin, glucose levels, induced diabetic, bodyweight. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Stz induced diabetes are commonly performed in male wistar kyoto rats. Diabetesinduced desensitization to ndmrs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of ndmrs. Streptozotocin stz is an alkylating agent that affects pancreatic islets, inducing diabetes in mice. Streptozotocin stz induced diabetes mellitus dm offers a very costeffective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. The advantages of nongenetic animal models of diabetes compared with the genetically.
Multiple low dose streptozotocin induced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Streptozotocin use in mice and rats version 1 date of publication. Effect of streptozotocin on glucose levels in albino wister rats. Multiple low dose streptozotocininduced diabetes in the rat. Streptozotocin stzinduced diabetes mellitus dm offers a very costeffective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. Full text streptozotocininduced type 1 diabetes in. Thirty white male sprague dawley rats weighing 150200 gm, about 4 month old were equally divided into the following. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic. Mrinmay das2, ashok kumar d1, mastanaiah k2 and arup das2. The most common animal model of human diabetes is streptozotocin stz induced diabetes in the rat. Comparative antidiabetic activity of methanolic extract of operculina turpethum stem and root against healthy and streptozotocin induced diabetic rats shankaraiah pulipaka1, srinivasa reddy challa2, ravindra babu pingili3 1trinity college of pharmaceutical sciences, peddapally, karimnagar, andhra pradesh, india, 505 172. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself.
Streptozotocininduced type 1 diabetes in rodents as a model. Induction of diabetes mellitus in rats using intraperitoneal streptozotocin. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute.
In conclusion, the streptozotocininduced diabetes mellitus in rats was stable for 6 months, afterward, however, a significant recovery developed in. The streptozotocin produced a stable form of diabetes, with blood changes consistent with the disease, and only minor. Diabetes mellitus is a metabolic disease, which progresses with chronic hyperglycaemia related to absolute and relative insulin deficiency, and it is also induced by genetic or environmental factors. Comparative antidiabetic activity of methanolic extract of. Longterm studies on pathological changes related to hyperglycaemia require a. Recognizing the signs of high and low blood sugar is crucial. Hyperglycemia enhances the production of reactive oxygen species, leading. What it means for you english, low literacy pdf very low literacy pdf. Therapeutic development to prevent bone loss andor restore lost bone in t1 diabetic patients requires knowledge of the molecular mechanisms accounting for the bone pathology. Streptozotocininduced diabetic ratsmice stz model advantages. Therefore, four groups of rats were allocated as control c, stzinduced dm d, and.
The blood glucose levels reached the maximum after 30 minutes of administration of 2 g glucosekg bw and then reduced to initial levels within 2 hours in all groups except diabetic rats that showed severe glucose intolerance throughout the experimental. At 99g body weight, diabetes was induced in some of the rats by intravenous administration of streptozotocin a gift of upjohn ltd. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Alhagi maurorum camel thorn plant is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. This project has assessed cardiovascular, ocular and neuropathic changes over a. The mmpc is a national institutes of healthsponsored resource that provides experimental testing services to scientists studying diabetes, obesity, diabetic. This protocol is used by diacomp members to induce diabetes in a number of the animal models developed by the consortium. If you are having difficulty downloading or saving the pdf, please refer to our pdf. Here, we thus combined highfathighfructose diet, with multiple lowdose injections of streptozotocin hf.
Streptozotocin, type i diabetes severity and bone katherine motyl and laura r. Streptozotocininduced experimental diabetes in male. Because cell culture models alone cannot fully address the systemicmetabolic complexity of t1 diabetes. To develop the corresponding animal pathology model, high fat diet hfd is often used. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. Jan 11, 2018 the epidemic of type 2 diabetes mellitus t2dm is fueled by added fructose consumption. Impact of streptozotocininduced diabetes and insulin therapy. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
Diabetes is induced in mice by using streptozotocin stz, a compound that has a preferential toxicity toward pancreatic. The resistance to ndmrs was stronger in the later stage of diabetes 16 versus 4 weeks after stz treatment. Autoxidation of dialuric acid generates superoxide radicals. Streptozotocininduced experimental diabetes in male wistar rats. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days, 60mgkg of streptozotocin was injected intravenously. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Streptozotocininduced type 1 diabetes in rodents as a. The streptozotocininduced diabetic nude mouse model.
Diabetes induced desensitization to ndmrs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of ndmrs. Effect of streptozotocin diabetes and insulin treatment on. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Detailed in this unit are protocols for producing stzinduced insulin deficiency and hyperglycemia in mice and rats.
Introduction diabetes mellitus, often simply referred to as diabetes. Diabetes resulted in impairment of growth rate and excretion of glucose in the urine. In a study with male icr mice, the effects of ursolic acid 0. Diabetes in humans induces chronic complications such as cardiovascular damage, cataracts and retinopathy, nephropathy and polyneuropathy. Induction of diabetes by streptozotocin in rats article pdf available in indian journal of clinical biochemistry 22222. Changes in oxidative stress and antioxidant enzyme. Effect of streptozotocin on glucose levels in albino. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. The timecourse changes in the blood glucose levels during the oral glucose tolerance test 0120 min of all groups were shown in figure 2. Streptozotocinhigh fat dietinduced type 2 diabetes bienta. After induction of diabetes, consumption of food and water and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body decreased in the diabetic animals. Three days after stz induction, the diabetes rats were treated with aqueous extract of erythrina variegata l. Carbohydrate metabolizing enzymes activity in streptozotocin.
At lower doses, stzinduced diabetes is not stable, since spontaneous recovery occurs. Streptozotocinhigh fat dietinduced type 2 diabetes. The mechanism of alloxan and streptozotocin action in b. Hypoglycemia low blood glucose diabetes education services.
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